4PGQ

Structure of human DNA polymerase beta complexed with G in the template base paired with incoming non-hydrolyzable TTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97648
Spacegroup name	P 1 21 1
Unit cell lengths	54.898, 80.087, 55.077
Unit cell angles	90.00, 106.15, 90.00

Refinement procedure

Resolution	19.268 - 2.300
R-factor	0.2061
Rwork	0.203
R-free	0.25950
RMSD bond length	0.006
RMSD bond angle	1.263
Data reduction software	DENZO
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.049
Number of reflections	28773
<I/σ(I)>	21.7
Completeness [%]	99.9
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	14%?23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5)