4PGM
SACCHAROMYCES CEREVISIAE PHOSPHOGLYCERATE MUTASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.467, 84.555, 88.884 |
| Unit cell angles | 90.00, 111.72, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pgm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 23.524 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.082 * | 0.253 |
| Number of reflections | 49635 | |
| <I/σ(I)> | 4.85 | 2.41 |
| Completeness [%] | 88.7 | 89.9 |
| Redundancy | 6.27 | 2.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.65 | PROTEIN WAS CRYSTALLISED FROM 22-24% PEG 4000 60MM TRIS-HCL, PH 8.65, 120MM LI2SO4, AND 1MM INOSITOL HEXAKISPHOSPHATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | inositol hexakisphosphate | 1 (mM) | |
| 3 | 1 | reservoir | Tris-HCl | 60 (mM) | pH8.65 |
| 4 | 1 | reservoir | lithium sulfate | 120 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 22-24 (%) |
