4PGJ

Human heavy-chain domain antibody in complex with hen egg-white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.954
Spacegroup name	I 1 2 1
Unit cell lengths	100.070, 40.310, 137.020
Unit cell angles	90.00, 101.20, 90.00

Refinement procedure

Resolution	49.080 - 2.600
R-factor	0.22788
Rwork	0.225
R-free	0.28660
RMSD bond length	0.015
RMSD bond angle	1.583
Refinement software	REFMAC (5.6.0111)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.080
High resolution limit [Å]	2.600
Rmerge	0.056
Number of reflections	16831
<I/σ(I)>	9
Completeness [%]	99.3
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	293	Protein at 4.4 mg/mL in 50 mM Tris (pH 7.5) amd 150 mM NaCl. Well solution contained 28% (w/v) PEG1500, 100 mM Na-citrate (pH 5.4). 400 nL of well solution and protein solution were combined.