4PGB
MHC Class I in complex with modified Sendai virus nucleoprotein peptide FAPGNWPAL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-17 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.97780 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.553, 89.257, 88.458 |
Unit cell angles | 90.00, 110.67, 90.00 |
Refinement procedure
Resolution | 44.629 - 2.800 |
R-factor | 0.2588 |
Rwork | 0.258 |
R-free | 0.28090 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.859 |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.629 | 44.629 | 2.950 |
High resolution limit [Å] | 2.799 | 8.850 | 2.800 |
Rmerge | 0.019 | 0.386 | |
Rmeas | 0.101 | ||
Rpim | 0.059 | 0.016 | 0.345 |
Total number of observations | 58044 | 1929 | 8293 |
Number of reflections | 23165 | ||
<I/σ(I)> | 10.4 | 29.7 | 2.3 |
Completeness [%] | 98.0 | 95.1 | 95.8 |
Redundancy | 2.5 | 2.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | sodium/potassium phosphate buffer 1.9M, MPD 1%, glycerol (cryoprotection) |