4PFU
Crystal structure of mannobiose bound oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (TM1226) from THERMOTOGA MARITIMA at 2.05 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-03-12 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 100.911, 193.867, 159.513 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.467 - 2.049 |
R-factor | 0.1752 |
Rwork | 0.173 |
R-free | 0.20810 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.073 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.073 |
High resolution limit [Å] | 2.049 | 2.049 |
Rmerge | 0.584 | |
Number of reflections | 96558 | |
<I/σ(I)> | 18.2 | 3.5 |
Completeness [%] | 98.5 | 96.1 |
Redundancy | 5.7 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 2.2 M AmSO4, 0.2 M tri-Li Citrate |