4PFP
Myosin VI motor domain in the Pi release state (with Pi) space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.650, 94.890, 97.300 |
| Unit cell angles | 90.00, 99.55, 90.00 |
Refinement procedure
| Resolution | 23.390 - 2.320 |
| R-factor | 0.1911 |
| Rwork | 0.189 |
| R-free | 0.22900 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.380 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.089 | 0.648 |
| Number of reflections | 78052 | |
| <I/σ(I)> | 14.44 | 2.27 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 6.67 | 3.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 4,5% PEG8k, 50mM MES pH 6,5, 1mM TCEP, 10mM NaH2PO4 |
