4PFE
Crystal structure of vsfGFP-0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 83.426, 83.426, 228.691 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.160 - 2.603 |
R-factor | 0.2013 |
Rwork | 0.199 |
R-free | 0.25530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k1k |
RMSD bond length | 0.004 |
RMSD bond angle | 1.045 |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.160 | 2.696 |
High resolution limit [Å] | 2.603 | 2.603 |
Rmerge | 0.112 | 0.603 |
Number of reflections | 25661 | |
<I/σ(I)> | 20.97 | 3.9 |
Completeness [%] | 100.0 | 99.48 |
Redundancy | 32 | 28.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1M Sodium acetate pH 4.6 30 %(v/v) PEG 400, 20mM Hepes pH 7.5, 150mM NaCl |