4PEX
Structure of the E502A variant of sacteLam55A from Streptomyces sp. SirexAA-E in complex with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.939, 100.055, 99.556 |
| Unit cell angles | 90.00, 92.31, 90.00 |
Refinement procedure
| Resolution | 24.265 - 1.750 |
| R-factor | 0.1423 |
| Rwork | 0.141 |
| R-free | 0.17210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pew |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.454 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.690 |
| High resolution limit [Å] | 1.660 | 4.500 | 1.660 |
| Rmerge | 0.082 | 0.033 | 0.546 |
| Total number of observations | 462173 | ||
| Number of reflections | 123726 | ||
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 99.9 | 98.6 | 100 |
| Redundancy | 3.7 | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Protein Solution (20 mg/ml protein, 0.05 M NaCl, and 0.010 M MOPS pH 7) mixed in a 1:1 ratio with the Well Solution (20% PEG 3350, 125mM NaCH02, and 100mM BTP pH 6.5). Cryoprotected with 20% PEG 3350, 125mM NaCH02, 75mM Glucose, 100mM BTP pH 6.5 and 15% ethylene glycol |
