4PER
Structure of Gallus gallus ribonuclease inhibitor complexed with Gallus gallus ribonuclease I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.662, 84.538, 121.661 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.815 - 1.920 |
R-factor | 0.2049 |
Rwork | 0.202 |
R-free | 0.25350 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.158 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.850 |
High resolution limit [Å] | 1.820 | 4.940 | 1.820 |
Rmerge | 0.134 | 0.032 | 0.687 |
Total number of observations | 201341 | ||
Number of reflections | 50875 | ||
<I/σ(I)> | 10.4 | ||
Completeness [%] | 99.4 | 94 | 99.8 |
Redundancy | 4 | 3.9 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 298 | 1:1 protein solution (20 mM HEPES-NaOH, 10 mM DTT, 2% w/v glycerol, pH 7.5) to well solution (100 mM citric acid, pH 3.5, 21% PEG1500), cryoprotectant: 100 mM citric acid, pH 3.5, 21% PEG1500, 15% ethylene glycol |