4PEI

Dbr1 in complex with synthetic branched RNA analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	73.037, 142.479, 214.665
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.370 - 1.950
R-factor	0.186
Rwork	0.184
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.498
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.110	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.097	0.510
Number of reflections	161620
<I/σ(I)>	9.1	2.1
Completeness [%]	99.1	100
Redundancy	4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	0.2 M lithium sulfate, 0.1 M bis-tris, 25% PEG 3350, 8.5 % glycerol, 2.0 mM nickel sulfate, 2.0 mM AK65