4PEG
Dbr1 in complex with guanosine-5'-monophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.2823 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.831, 141.499, 212.942 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.538 - 2.000 |
R-factor | 0.1989 |
Rwork | 0.197 |
R-free | 0.22910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.902 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.840 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.499 | |
Number of reflections | 143800 | |
<I/σ(I)> | 13 | 3.1 |
Completeness [%] | 96.7 | 96.4 |
Redundancy | 4.9 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.2 M lithium sulfate, 0.1 M bis-tris, 25% PEG 3350, 8.5 % glycerol, 1.0 mM guanosine-5'-monophosphate, 2.0 mM manganese sulfate |