4PDR
Crystal Structure of a K+ selective NaK mutant in Barium and Sodium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 78 |
Detector technology | CCD |
Collection date | 2013-11-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9999 |
Spacegroup name | I 4 |
Unit cell lengths | 68.042, 68.042, 89.627 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.810 - 1.850 |
R-factor | 0.201 |
Rwork | 0.200 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ouf |
RMSD bond length | 0.008 |
RMSD bond angle | 1.091 |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.800 |
Number of reflections | 18778 |
<I/σ(I)> | 39.2 |
Completeness [%] | 99.2 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside |