4PDP
Crystal structure of Rad53 kinase domain and SCD2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.430, 79.070, 227.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.855 - 2.591 |
| R-factor | 0.2296 |
| Rwork | 0.227 |
| R-free | 0.26110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ig1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.613 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.890 | 2.700 |
| High resolution limit [Å] | 2.591 | 2.591 |
| Rmerge | 0.067 | 0.153 |
| Number of reflections | 21728 | |
| <I/σ(I)> | 15.3 | 2.4 |
| Completeness [%] | 94.5 | 70.2 |
| Redundancy | 6.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% v/v PEG400, 50 mM sodium citrate, 100 mM Tris, pH 8.0 |
