4PDO

Structure of Ephrin type-A receptor 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 32
Unit cell lengths	92.461, 92.461, 75.836
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.902 - 2.104
R-factor	0.1615
Rwork	0.160
R-free	0.18380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mqb
RMSD bond length	0.007
RMSD bond angle	1.016
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	15.000	15.000	2.140
High resolution limit [Å]	2.100	5.600	2.100
Rmerge	0.086	0.051	0.444
Total number of observations	197356
Number of reflections	41909
<I/σ(I)>	9.3
Completeness [%]	99.8	97.6	100
Redundancy	4.7	4	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.8 M Na/K L(+)-tartrate, 0.1 M Tris-Cl pH 8.5, 1% (w/v) PEGMME 5000