4PDG

Crystal structure of a complex between an inhibited LlFpg and a THF containing DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-07-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98011
Spacegroup name	P 41 21 2
Unit cell lengths	90.550, 90.550, 139.353
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.060 - 2.400
R-factor	0.1647
Rwork	0.163
R-free	0.19250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pm5
RMSD bond length	0.012
RMSD bond angle	1.281
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.060	2.530
High resolution limit [Å]	2.400	2.400
Rmerge		0.871
Number of reflections	43014
<I/σ(I)>	16.2	2
Completeness [%]	99.1	99.5
Redundancy	4.8	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	HEPES, SODIUM CITRATE