4PD3

Crystal Structure of Rigor-Like Human Nonmuscle Myosin-2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-29
Detector	PSI PILATUS 6M
Wavelength(s)	0.976300
Spacegroup name	P 1 21 1
Unit cell lengths	65.850, 157.870, 143.480
Unit cell angles	90.00, 94.21, 90.00

Refinement procedure

Resolution	29.998 - 2.840
R-factor	0.2543
Rwork	0.253
R-free	0.28820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.710
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.840
Number of reflections	65526
<I/σ(I)>	7.21
Completeness [%]	95.1
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	291.15	10% PEG-6000, 0.1 M Tris pH 7.5