4PD2
Crystal structure of a complex between a C248GH LlFpg mutant and a THF containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98400 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 91.456, 91.456, 140.389 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.728 - 1.650 |
R-factor | 0.1538 |
Rwork | 0.152 |
R-free | 0.17890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pm5 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.680 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.560 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.058 | 0.705 |
Number of reflections | 70733 | |
<I/σ(I)> | 14.2 | 2.2 |
Completeness [%] | 96.1 | 96.4 |
Redundancy | 4.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | HEPES, SODIUM CITRATE |