4PD2

Crystal structure of a complex between a C248GH LlFpg mutant and a THF containing DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98400
Spacegroup name	P 41 21 2
Unit cell lengths	91.456, 91.456, 140.389
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.728 - 1.650
R-factor	0.1538
Rwork	0.152
R-free	0.17890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pm5
RMSD bond length	0.017
RMSD bond angle	1.680
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.560	1.740
High resolution limit [Å]	1.650	1.650
Rmerge	0.058	0.705
Number of reflections	70733
<I/σ(I)>	14.2	2.2
Completeness [%]	96.1	96.4
Redundancy	4.9	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	HEPES, SODIUM CITRATE