4PD1
Structure of gephyrin E domain with Glycine-beta receptor peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9184 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 85.660, 100.060, 117.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.472 - 1.975 |
R-factor | 0.1605 |
Rwork | 0.159 |
R-free | 0.19550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fu3 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.297 |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.472 |
High resolution limit [Å] | 1.975 |
Rmerge | 0.111 |
Number of reflections | 35783 |
<I/σ(I)> | 17.7 |
Completeness [%] | 100.0 |
Redundancy | 14.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 M sodium citrate, pH 4.5, 28-34 % 2-methyl-2-4-pentanediol |