4PD0

1.7 A resolution structure of gephyrin's E-domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9198
Spacegroup name	I 2 2 2
Unit cell lengths	87.655, 100.008, 113.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.828 - 1.700
R-factor	0.1618
Rwork	0.161
R-free	0.18420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fu3
RMSD bond length	0.011
RMSD bond angle	1.322
Data scaling software	Aimless
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	43.830
High resolution limit [Å]	1.700
Rmerge	0.105
Number of reflections	55179
<I/σ(I)>	15
Completeness [%]	100.0
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.1 M sodium-citrate, pH 4.5, 28-34% 2-methyl-2,4-pentanediol