4PD0
1.7 A resolution structure of gephyrin's E-domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9198 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 87.655, 100.008, 113.695 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.828 - 1.700 |
R-factor | 0.1618 |
Rwork | 0.161 |
R-free | 0.18420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fu3 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.322 |
Data scaling software | Aimless |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.830 |
High resolution limit [Å] | 1.700 |
Rmerge | 0.105 |
Number of reflections | 55179 |
<I/σ(I)> | 15 |
Completeness [%] | 100.0 |
Redundancy | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 M sodium-citrate, pH 4.5, 28-34% 2-methyl-2,4-pentanediol |