4PCZ
Crystal structure of a complex between R247G LlFPG mutant and a THF containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98011 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 91.723, 91.723, 142.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.940 - 1.700 |
R-factor | 0.158 |
Rwork | 0.157 |
R-free | 0.18150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pm5 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.825 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.940 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.095 | 0.411 |
Number of reflections | 65739 | |
<I/σ(I)> | 8.4 | 2.6 |
Completeness [%] | 97.4 | 88.2 |
Redundancy | 3.6 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | HEPES, SODIUM CITRATE |