4PCV
The structure of BdcA (YjgI) from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 131.058, 52.461, 69.807 |
Unit cell angles | 90.00, 118.06, 90.00 |
Refinement procedure
Resolution | 47.800 - 2.050 |
R-factor | 0.2017 |
Rwork | 0.201 |
R-free | 0.22180 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.591 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 26365 | |
<I/σ(I)> | 14.3 | 2.9 |
Completeness [%] | 99.3 | 99.2 |
Redundancy | 3.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 50% (v/v) PEG-200, 0.1 M Tris pH 7.0, 0.05 M LiSO4 |