4PCV
The structure of BdcA (YjgI) from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.058, 52.461, 69.807 |
| Unit cell angles | 90.00, 118.06, 90.00 |
Refinement procedure
| Resolution | 47.800 - 2.050 |
| R-factor | 0.2017 |
| Rwork | 0.201 |
| R-free | 0.22180 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.591 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 26365 | |
| <I/σ(I)> | 14.3 | 2.9 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 50% (v/v) PEG-200, 0.1 M Tris pH 7.0, 0.05 M LiSO4 |
