4PCO
Crystal structure of double-stranded RNA with four terminal GU wobble base pairs
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795, 1.3624, 1.6053, 1.6068 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 36.616, 43.224, 83.256 |
Unit cell angles | 90.00, 102.48, 90.00 |
Refinement procedure
Resolution | 27.521 - 1.320 |
R-factor | 0.1567 |
Rwork | 0.155 |
R-free | 0.18350 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.038 |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | PHENIX (1.9_1678) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 81.289 | 27.521 | 1.390 |
High resolution limit [Å] | 1.320 | 4.170 | 1.320 |
Rmerge | 0.045 | 0.667 | |
Rmeas | 0.070 | ||
Rpim | 0.023 | 0.017 | 0.289 |
Total number of observations | 236794 | 10406 | 31195 |
Number of reflections | 29968 | ||
<I/σ(I)> | 18.2 | 61.4 | 2.7 |
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 7.9 | 10.5 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 15 mg/mL RNA, 26.2% (v/v) 2-methyl-2,4-pentanediol (MPD), 0.04 M lithium chloride, 0.03 M cobalt hexamine chloride, 0.04 M sodium cacodylate, pH 5.5 |