4PCG
Structure of Human Polyomavirus 6 (HPyV6) VP1 pentamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 183.900, 89.440, 125.270 |
Unit cell angles | 90.00, 131.26, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.14926 |
Rwork | 0.148 |
R-free | 0.17438 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Human Polyomavirus 7 (HPyV7) VP1 pentamer |
RMSD bond length | 0.009 |
RMSD bond angle | 1.381 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 140450 | |
<I/σ(I)> | 14.8 | 2.8 |
Completeness [%] | 99.2 | 96.4 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris pH 8.5, 200 mM NaSCN, 13.3% (w/v) PEG 3350 |