4PC8
Structure-based protein engineering efforts on the scaffold of a monomeric triosephosphate isomerase yielding a sugar isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.620, 62.620, 136.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.880 - 1.550 |
| R-factor | 0.17546 |
| Rwork | 0.173 |
| R-free | 0.21649 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.683 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.880 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 38092 | |
| <I/σ(I)> | 23.9 | 1.9 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 12.3 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 21% PEG6000, 100mM citric acid pH5.5, 5% glycerol, 100mM L-arabinose (no further pH adjustment) |
