4PC4

Bombyx mori lipoprotein 6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 1 21 1
Unit cell lengths	83.210, 85.750, 104.470
Unit cell angles	90.00, 104.84, 90.00

Refinement procedure

Resolution	47.000 - 1.800
R-factor	0.16147
Rwork	0.161
R-free	0.18264
RMSD bond length	0.013
RMSD bond angle	1.528
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.050	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.048	0.550
Number of reflections	123486
<I/σ(I)>	22.32	3.48
Completeness [%]	94.9	73
Redundancy	6.44	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	10% isopropanol, 10% PEG 3350, 0.1 M MES