4PC1
Elongation Factor Tu:Ts complex with a bound phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.5 |
Synchrotron site | SRS |
Beamline | PX9.5 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-08-10 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9215 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.510, 109.770, 195.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.786 - 1.950 |
R-factor | 0.1757 |
Rwork | 0.174 |
R-free | 0.20770 |
Starting model (for MR) | 1tui |
RMSD bond length | 0.013 |
RMSD bond angle | 1.350 |
Refinement software | PHENIX ((phenix.refine: dev_1593)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.800 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.658 | |
Number of reflections | 118029 | |
<I/σ(I)> | 11.6 | 1.8 |
Completeness [%] | 96.8 | 97.2 |
Redundancy | 2.7 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 100 mM Hepes,22% PEG 6000, 150mM NaCl, 10% glycerol |