4PB6
Feline calicivirus VP1 T=1 virus-like particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9185 |
Spacegroup name | P 21 3 |
Unit cell lengths | 358.336, 358.336, 358.336 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.510 - 8.000 |
R-factor | 0.44232 |
Rwork | 0.441 |
R-free | 0.46285 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdb 3m8l and model builting |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.200 | 8.430 |
High resolution limit [Å] | 8.000 | 8.000 |
Rmerge | 0.240 | 1.100 |
Number of reflections | 14809 | |
<I/σ(I)> | 3.07 | 0.69 |
Completeness [%] | 87.2 | 61.5 |
Redundancy | 5.5 | 1.82 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | reservoir solution: 7 - 10 % PEG 6000, 0.1 M Hepes pH 7, 0.5 - 1 M LiCl protein in: 20 mM MES pH 6, 200 mM NaCl at 3.4 mg/ml |