4PAS

Heterodimeric coiled-coil structure of human GABA(B) receptor

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97898,0.97934,0.96863
Spacegroup name	C 2 2 21
Unit cell lengths	46.721, 54.654, 55.347
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.510 - 1.620
R-factor	0.1938
Rwork	0.193
R-free	0.21710
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	0.827
Data reduction software	HKL-2000
Refinement software	REFMAC (5.4.0066)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.680
High resolution limit [Å]	1.620	3.490	1.620
Rmerge	0.036	0.035	0.055
Total number of observations	53628
Number of reflections	8951
<I/σ(I)>	43.8		17.79
Completeness [%]	95.8	92.7	76.3
Redundancy	6	6.3	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	3.0M ammonium sulphate, 0.1M bicine, pH9.0