4PAG
ABC transporter solute binding protein from Sulfurospirillum deleyianum DSM 6946
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.055, 50.813, 91.544 |
| Unit cell angles | 90.00, 98.00, 90.00 |
Refinement procedure
| Resolution | 40.210 - 1.901 |
| R-factor | 0.1445 |
| Rwork | 0.141 |
| R-free | 0.20230 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.199 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.119 | 0.058 | 0.960 |
| Total number of observations | 426232 | ||
| Number of reflections | 59880 | ||
| <I/σ(I)> | 6.8 | ||
| Completeness [%] | 99.9 | 99.1 | 99.9 |
| Redundancy | 7.1 | 6.9 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | magnesiun chloride, MES, PEG4000 |
