4PA6
Structure of NavMS pore and C-terminal domain crystallised in the presence of channel blocking compound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.915 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.934, 324.571, 79.766 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.770 - 3.360 |
| R-factor | 0.3041 |
| Rwork | 0.302 |
| R-free | 0.34210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | using 3ZJZ as starting model |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.770 | 3.630 |
| High resolution limit [Å] | 3.360 | 3.360 |
| Rmerge | 0.184 | 0.622 |
| Number of reflections | 15259 | |
| <I/σ(I)> | 12 | 4.4 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 13.1 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1 M Sodium ctirate, 0.1 M TRIS, pH 8.0, 34 % PEG |
