4P9U
FadR, Fatty Acid Responsive Transcription Factor from Vibrio cholerae, in Complex with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-02-21 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0781 |
Spacegroup name | P 1 |
Unit cell lengths | 84.600, 94.700, 101.700 |
Unit cell angles | 89.80, 114.60, 116.50 |
Refinement procedure
Resolution | 19.888 - 3.208 |
R-factor | 0.2193 |
Rwork | 0.218 |
R-free | 0.25230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p96 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.357 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER ((phenix.molecular replacement: 1.8.4_1496)) |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.890 | 3.322 |
High resolution limit [Å] | 3.208 | 3.208 |
Number of reflections | 40186 | |
<I/σ(I)> | 12.97 | 3 |
Completeness [%] | 98.4 | 95.86 |
Redundancy | 3.51 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2 M lithium sulfate, 0.1 M TRIS pH 8.5, 1.26 M ammonium sulfate |