4P9J

Crystal Structure of rabbit Ryanodine Receptor 1 SPRY2 Domain (1070-1246)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03
Spacegroup name	P 1
Unit cell lengths	37.580, 37.570, 95.820
Unit cell angles	89.97, 89.97, 120.05

Refinement procedure

Resolution	32.549 - 1.840
R-factor	0.1661
Rwork	0.163
R-free	0.21970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.357
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX ((phenix.refine: dev_1637))

Data quality characteristics

	Overall
Low resolution limit [Å]	32.550
High resolution limit [Å]	1.840
Number of reflections	36943
<I/σ(I)>	8.1
Completeness [%]	94.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	sodium citrate