4P9E
Crystal structure of dCMP deaminase from the cyanophage S-TIM5 in apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9763 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 78.130, 78.130, 80.560 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 67.660 - 2.600 |
R-factor | 0.21466 |
Rwork | 0.212 |
R-free | 0.27163 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hvv |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.070 | 0.283 |
Number of reflections | 4840 | |
<I/σ(I)> | 23 | 8.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.7 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.8M Malic acid pH 7 |