4P9C
Crystal structure of dCMP deaminase from the cyanophage S-TIM5 in complex with dCMP and dUMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9322 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.050, 147.210, 100.470 |
| Unit cell angles | 90.00, 95.62, 90.00 |
Refinement procedure
| Resolution | 99.990 - 2.600 |
| R-factor | 0.23257 |
| Rwork | 0.231 |
| R-free | 0.26783 |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.990 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Completeness [%] | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MMT buffer pH 8, 25% (w/v) PEG 1500, 5mM Magnesium chloride and 5mM dCTP |
