4P9A

X-ray Crystal Structure of PA protein from Influenza strain H7N9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-20
Detector	RAYONIX MX-225
Wavelength(s)	0.97872
Spacegroup name	P 65 2 2
Unit cell lengths	68.890, 68.890, 400.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.653 - 2.200
R-factor	0.204
Rwork	0.203
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2znl
RMSD bond length	0.008
RMSD bond angle	0.975
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.260
High resolution limit [Å]	2.200	9.840	2.200
Rmerge	0.057	0.022	0.585
Rmeas	0.063	0.025	0.641
Number of reflections	30002	399	2161
<I/σ(I)>	19.93	51.02	3.1
Completeness [%]		90.7	100
Redundancy	5.87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	400 nl of protein solution at 20.77 mg/ml plus 400 nl precipitant solution equilibrated against 80 uL of precipitant. Precipitant: Wizard 3/4 well A10 - 200 mM sodium thiocyanate, 20% PEG3350