4P97
Functional conservation despite structural divergence in ligand-responsive RNA switches
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 173 |
Detector technology | IMAGE PLATE |
Collection date | 2014-02-03 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | H 3 |
Unit cell lengths | 60.532, 60.532, 122.679 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.210 - 1.860 |
R-factor | 0.21163 |
Rwork | 0.212 |
R-free | 0.19682 |
Structure solution method | MOLECULAR REPLACEMENT |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.560 | 1.930 |
High resolution limit [Å] | 1.860 | 1.860 |
Number of reflections | 1699 | |
<I/σ(I)> | 10.78 | 3.67 |
Completeness [%] | 99.1 | 90.3 |
Redundancy | 8.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 24% PEG 4000, 10mM Calcium chloride, 200 mM Ammonium Chloride |