4P96
FadR, Fatty Acid Responsive Transcription Factor from Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-02-11 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9764 |
Spacegroup name | P 41 |
Unit cell lengths | 87.480, 87.480, 81.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.560 - 2.200 |
R-factor | 0.1759 |
Rwork | 0.174 |
R-free | 0.20460 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.892 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX ((phenix.molecular replacement: 1.8.4-1496)) |
Refinement software | PHENIX ((phenix.refine: 1.8.4-1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.560 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 31200 | |
<I/σ(I)> | 33.79 | 6.62 |
Completeness [%] | 100.0 | 100 |
Redundancy | 15.25 | 15.22 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2 M sodium chloride, 0.1 M MES pH 6.5, 10%(w/v) PEG 4000 |