4P91

Crystal structure of the nogo-receptor-2 (27-330)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	56.759, 129.155, 57.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.100
R-factor	0.16031
Rwork	0.158
R-free	0.19528
RMSD bond length	0.026
RMSD bond angle	2.329
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge		0.442
Number of reflections	23662
<I/σ(I)>	19.4	2.6
Completeness [%]	98.6	89.5
Redundancy	2.7	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.6 M KNAPO4