4P8I
Tgl - a bacterial spore coat transglutaminase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-11 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.918 |
Spacegroup name | P 41 |
Unit cell lengths | 97.940, 97.940, 66.183 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.850 - 1.850 |
Rwork | 0.158 |
R-free | 0.20800 |
Data scaling software | XDS (31-12-2013) |
Phasing software | SHARP (2.8.2) |
Refinement software | BUSTER-TNT (2.10) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.850 |
High resolution limit [Å] | 1.850 |
Number of reflections | 47409 |
<I/σ(I)> | 29.53 |
Completeness [%] | 88.6 |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 41% (w/V) PEG 400, 0.2 M lithium sulphate, 0.1 M sodum citrate |