4P8E
Structure of ribB complexed with substrate (Ru5P) and metal ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-06-26 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.079, 77.090, 97.531 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.040 |
R-factor | 0.17844 |
Rwork | 0.176 |
R-free | 0.23216 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g58 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.147 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.160 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.065 | 0.258 |
Number of reflections | 25330 | |
<I/σ(I)> | 23.1 | 6.4 |
Completeness [%] | 98.3 | 92.7 |
Redundancy | 7.3 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.1 | 293 | PEG3350, Na2HPO4 |