4P87
Crystal structure of Est-Y29, a novel penicillin-binding protein/beta-lactamase homolog from a metagenomic library
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | I 4 |
Unit cell lengths | 121.731, 121.731, 155.829 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.000 - 1.999 |
R-factor | 0.17944 |
Rwork | 0.178 |
R-free | 0.21224 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.247 |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.999 |
Number of reflections | 76224 |
<I/σ(I)> | 28.1 |
Completeness [%] | 99.8 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 287 | 1 M sodium citrate and 100 mM sodium acetate, pH 4.6 |