4P7D
Antitoxin HicB3 crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.840, 76.870, 118.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.846 - 2.781 |
| R-factor | 0.209 |
| Rwork | 0.205 |
| R-free | 0.28240 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.431 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.850 | 2.950 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.095 | 0.683 |
| Number of reflections | 16491 | |
| <I/σ(I)> | 14.45 | 2.84 |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | polyethylene glycol 3000, MES pH 6.5 |
