4P7B
Crystal structure of S. typhimurium peptidyl-tRNA hydrolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.097, 64.925, 110.489 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.876 - 1.600 |
R-factor | 0.1749 |
Rwork | 0.174 |
R-free | 0.19760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fyj |
RMSD bond length | 0.012 |
RMSD bond angle | 1.285 |
Refinement software | PHENIX ((phenix.refine: dev_1458)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.880 |
High resolution limit [Å] | 1.600 |
Number of reflections | 59556 |
<I/σ(I)> | 16.57 |
Completeness [%] | 99.8 |
Redundancy | 5.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 291 | citric acid, Bis-Tris propane, glycerol, sucrose, PEG6000 |