4P71
Apo PheRS from P. aeuriginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-12 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.780, 219.080, 107.130 |
| Unit cell angles | 90.00, 101.91, 90.00 |
Refinement procedure
| Resolution | 39.000 - 2.790 |
| R-factor | 0.1931 |
| Rwork | 0.191 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rhq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.069 | 0.698 |
| Number of reflections | 62453 | |
| <I/σ(I)> | 10.2 | 4.1 |
| Completeness [%] | 98.8 | 92.4 |
| Redundancy | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 18% PEG 3350, 0.2M ammonium citrate tri-basic (pH 6.2) |
