4P6T
Crystal Structure of tyrosinase from Bacillus megaterium with p-tyrosol in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-04-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.690, 78.090, 84.280 |
| Unit cell angles | 90.00, 105.96, 90.00 |
Refinement procedure
| Resolution | 56.230 - 2.500 |
| Rwork | 0.208 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 81.031 | 56.229 | 2.640 |
| High resolution limit [Å] | 2.500 | 7.910 | 2.500 |
| Rmerge | 0.089 | 0.240 | |
| Rmeas | 0.134 | ||
| Rpim | 0.076 | 0.062 | 0.162 |
| Total number of observations | 60015 | 2000 | 8444 |
| Number of reflections | 20094 | ||
| <I/σ(I)> | 7.3 | 11.1 | 3.9 |
| Completeness [%] | 97.4 | 97.4 | 95.5 |
| Redundancy | 3 | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG8000, sodium cacodylate |
