4P6Q

The crystal structure of the Split End protein SHARP adds a new layer of complexity to proteins containing RNA Recognition Motifs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM16
Synchrotron site	ESRF
Beamline	BM16
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	60.670, 69.710, 88.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.710 - 2.000
R-factor	0.1959
Rwork	0.192
R-free	0.23770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Ensemble 1 (PDB codes: 3MD1 2adc 2dnm 2dgu 2CQB); Ensemble 2 (PDB codes: X4AR 2ytc 4f26 1why 2CPZ); Ensemble 3 (PDB codes: 2I38 1why 1x55 2lcw 2CPE).
RMSD bond length	0.008
RMSD bond angle	1.096
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.710
High resolution limit [Å]	2.000	2.000
Number of reflections	26164
<I/σ(I)>	20.44
Completeness [%]	99.7	100
Redundancy	5.38	5.63

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	15% PEG 8000, 0.1M ammonium sulphate, 0.01M magnesium chloride, 0.05M 2-(N-morpholino)ethanesulfonic acid