4P6F
Crystal structure of the peptolide 12C bound to bacterial ribosome
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.240, 443.460, 618.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.792 - 3.600 |
R-factor | 0.2237 |
Rwork | 0.222 |
R-free | 0.25420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2WDG 2WDH 2WDI 2WDJ |
RMSD bond length | 0.006 |
RMSD bond angle | 0.887 |
Data reduction software | HKL-2000 |
Data scaling software | XSCALE |
Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.700 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 1.169 | |
Number of reflections | 638335 | |
<I/σ(I)> | 7.6 | 1.8 |
Completeness [%] | 98.0 | 95.8 |
Redundancy | 7.4 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M Tris-Acetate, 0.2M KSCN, 10 mM MgCl2 |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M Tris-Acetate, 0.2M KSCN, 10 mM MgCl2 |
2 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M Tris-Acetate, 0.2M KSCN, 10 mM MgCl2 |
2 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M Tris-Acetate, 0.2M KSCN, 10 mM MgCl2 |