4P5S
Structure of reduced W45Y mutant of amicyanin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.165, 56.646, 28.663 |
| Unit cell angles | 90.00, 96.67, 90.00 |
Refinement procedure
| Resolution | 28.000 - 1.020 |
| R-factor | 0.135 |
| Rwork | 0.134 |
| R-free | 0.15000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Native amicyanin (1AAC) |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.854 |
| Data reduction software | HKL-2000 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.060 |
| High resolution limit [Å] | 1.020 | 1.020 |
| Rmerge | 0.044 | 0.136 |
| Number of reflections | 40243 | |
| <I/σ(I)> | 34.1 | 11.8 |
| Completeness [%] | 91.7 | 76.1 |
| Redundancy | 5.9 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | Initial crystals were obtained from Hampton research ammonium sulfate screen (HR2-211). Bigger crystals grown by macro-seeding using 3.2M Na/K phosphate solution. |
