4P5P

X-ray structure of Francisella tularensis Rapid Encystment Protein 24 KDa (REP24), gene product of FTN_0841

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1159
Spacegroup name	P 32 2 1
Unit cell lengths	44.439, 44.439, 183.454
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.486 - 1.540
R-factor	0.1495
Rwork	0.147
R-free	0.19350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u9c
RMSD bond length	0.007
RMSD bond angle	1.114
Data reduction software	DENZO
Data scaling software	SCALA (3.2.5)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	61.151	91.670	1.580
High resolution limit [Å]	1.500	4.740	1.500
Rmerge	0.095	0.056	0.226
Total number of observations	237816	10242	12964
Number of reflections	33819
<I/σ(I)>	13.4	26.2	1.9
Completeness [%]	96.9	99.8	82.9
Redundancy	7	8	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	7.1% (w/v) PEG6000, 0.1M Na-Succinate pH 5.5, 0.58% Heptanoyl-N-methylglucamideMG7