4P5N

Structure of CNAG_02591 from Cryptococcus neoformans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	31.775, 34.686, 38.253
Unit cell angles	73.93, 72.85, 79.46

Refinement procedure

Resolution	35.510 - 1.490
R-factor	0.1392
Rwork	0.137
R-free	0.18020
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.302
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.520
High resolution limit [Å]	1.490	4.040	1.490
Rmerge	0.048	0.037	0.147
Total number of observations	64741
Number of reflections	23265
<I/σ(I)>	18.8		9.88
Completeness [%]	95.7	91.9	91.6
Redundancy	2.8	2.6	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M cadmium sulfate, 2.2M ammonium sulfate